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Prosomes discriminate between mRNA of adenovirus-infected and uninfected HeLa cells
A Horsch1, C Martins de Sa, B Dineva
1Biologisches Institut, Universität Stuttgart, FRG.
Abstract:
Prosomes are small cytoplasmic RNP complexes associated with repressed mRNA. In in vitro translation, they discriminate between the mRNA of adenovirus-infected HeLa cells and those of uninfected cells grown under normal conditions. Prosomes as well as their RNA constituents interact much more strongly with poly(A)+ mRNA of infected cells and inhibit their translation in vitro preferentially. A possible role of prosomes in the differential regulation of translation is discussed.
Insights
Prosomes, small RNP complexes, preferentially inhibit translation of adenovirus-infected cell mRNA. They bind more strongly to infected cell poly(A)+ mRNA, suggesting a role in differential gene regulation.
Area of Science:
- Molecular Biology
- Cell Biology
- Virology
Background:
- Prosomes are cytoplasmic ribonucleoprotein (RNP) complexes implicated in mRNA regulation.
- Differential gene expression is crucial for cellular processes, including viral infection responses.
Purpose of the Study:
- To investigate the role of prosomes in the differential regulation of mRNA translation.
- To determine if prosomes discriminate between mRNA from infected and uninfected cells.
Main Methods:
- In vitro translation assays using mRNA from adenovirus-infected and uninfected HeLa cells.
- Analysis of prosome-mRNA interactions, particularly with poly(A)+ mRNA.
Main Results:
- Prosomes exhibit discriminatory binding to mRNA, interacting more strongly with poly(A)+ mRNA from infected cells.
- Prosomes preferentially inhibit the in vitro translation of mRNA from adenovirus-infected cells.
Conclusions:
- Prosomes play a role in the selective regulation of mRNA translation.
- Prosomes may contribute to the host cell's response to viral infection by modulating specific mRNA translation.