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Published on: August 12, 2015
[Detection of Bcl-2 in human hepatocellular carcinoma and down-regulation with siRNA]
Zhikui Liu1, Tao Song1, Changwei Dou1
1Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Objective:
To investigate the relationship between the level of Bcl-2 in human hepatocellular carcinoma (HCC) tissues and the clinicopathological characteristics and prognosis of patients, and to detect the proliferation of SMMC-7721 cells after Bcl-2 is down-regulated with siRNA.
Methods:
The level of Bcl-2 in 68 HCC tissues was detected by reverse transcription PCR, SP immunohistochemistry and Western blotting. The 68 HCC tissues were divided into two groups based on the level of Bcl-2. The clinicopathological characteristics and prognosis of the patients were compared between groups. The proliferation of SMMC-7721 cells was evaluated by MTT assay after Bcl-2 was inhibited with siRNA.
Results:
Compared with corresponding noncancerous liver tissues, the level of Bcl-2 was significantly elevated in HCC tissues. The level of Bcl-2 was correlated with clinicopathological characteristics, and the patient with higher expression level of Bcl-2 had relatively poorer prognosis. Down-regulation of Bcl-2 expression by siRNA significantly inhibited the proliferation of SMMC-7721 cells.
Conclusion:
The level of Bcl-2 is abnormally elevated in HCC tissues and can serve as a predictor of the prognosis of patients with HCC. The down-regulated of Bcl-2 in SMMC-7721 cells can inhibit the proliferation of cancer cells.
