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Updated: Apr 17, 2026
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
Quantitative analysis of plasma proteins in whole blood-derived fresh frozen plasma prepared with three pathogen
Luis Larrea1, María-Isabel Ortiz-de-Salazar1, Patricia Martínez1
1Servicio de Fraccionamiento, Centro de Transfusión de la Comunidad Valenciana, Avenida del Cid 65, 46014 Valencia, Spain.
Abstract:
Several plasma pathogen reduction technologies (PRT) are currently available. We evaluated three plasma PRT processes: Cerus Amotosalen (AM), Terumo BCT riboflavin (RB) and Macopharma methylene blue (MB). RB treatment resulted in the shortest overall processing time and in the smallest volume loss (1%) and MB treatment in the largest volume loss (8%). MB treatment retained the highest concentrations of factors II, VII, X, IX, Protein C, and Antithrombin and the AM products of factor V and XI. Each PRT process evaluated offered distinct advantages such as procedural simplicity and volume retention (RB) and overall plasma protein retention (MB).
