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An assay for dihydroorotase using high-performance liquid chromatography with radioactivity detection
1Merrell Dow Research Institute, Cincinnati, Ohio 45215.
Analytical Biochemistry
|January 1, 1989
Abstract:
An assay for measuring dihydroorotase activity was devised. Radiolabeled substrate and product were separated by high-performance liquid chromatography using a reverse-phase column with ion-pairing, and the radioactivity was quantitated by flow detection.