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Related Experiment Video

Updated: Apr 17, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

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A combinatorial bidirectional and bicistronic approach for coordinated multi-gene expression in corn.

Sandeep Kumar¹, Diaa AlAbed, John T Whitteck

¹Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, IN, 46268, USA, Skumar9@dow.com.

Plant Molecular Biology

|February 7, 2015

Summary

This summary is machine-generated.

Researchers developed a novel gene stacking strategy in corn using a single bidirectional promoter (BDP) to co-regulate multiple genes. This approach enhances coordinated gene expression for complex trait development in crops.

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Published on: July 23, 2014

Area of Science:

Plant biotechnology
Molecular genetics
Crop improvement

Background:

Transgene stacking for trait development is increasingly complex due to multiple desired traits and modes of action.
Coordinated expression of multiple transgenes is crucial for efficient trait development in genetically engineered crops.

Purpose of the Study:

To develop and demonstrate a novel gene stacking strategy in corn using a single promoter for co-regulation of multiple genes.
To combine bidirectional promoter (BDP) and bicistronic approaches for coordinated multi-gene expression.

Main Methods:

Conversion of a unidirectional Zea mays Ubiquitin-1 (ZMUbi1) promoter into a synthetic BDP.
Integration of a bicistronic organization with Thosea asigna virus 2A auto-cleaving domains at both ends of the BDP.
Generation of transgenic corn expressing four transgenes (insect resistance: cry34Ab1, cry35Ab1; herbicide resistance: aad1; reporter: phiYFP) using the single ZMUbi1 BDP.

Main Results:

Successful expression of four transgenes in transgenic corn using a single ZMUbi1 BDP.
Gene expression analyses confirmed coordinated expression of the four genes.
Demonstrated superior coordinated expression compared to constructs with independent unidirectional ZmUbi1 promoters.

Conclusions:

This study presents the first report of a single promoter for co-regulating multiple genes in a crop plant.
The developed stacking technology offers a valuable tool for engineering metabolic pathways in both basic and applied research.
This approach simplifies complex genetic engineering strategies for crop improvement.