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Complete Genome of Agrobacterium fabrum Strain 1D1416
Diaa Alabed1, Naxin Huo1,2, Yong Gu1
1USDA-ARS Crop Improvement and Genetics, Western Regional Research Center, Albany, California, USA.
Microbiology Resource Announcements
|June 20, 2023
Summary
The Agrobacterium fabrum 1D1416 genome was sequenced, revealing its genetic makeup including chromosomes and plasmids. This nondisarmed strain causes gall-like structures in citrus plants.
Area of Science:
- Microbiology
- Genomics
- Plant Pathology
Background:
- Agrobacterium fabrum is a plant pathogen known for its ability to induce tumors.
- Understanding the genetic basis of Agrobacterium virulence is crucial for developing control strategies.
Purpose of the Study:
- To report the draft genome sequence of Agrobacterium fabrum strain 1D1416.
- To characterize the genomic components, including chromosomes and plasmids, of this strain.
Main Methods:
- Whole-genome sequencing of Agrobacterium fabrum strain 1D1416.
- Genome assembly and annotation.
Main Results:
- The assembled genome consists of a circular chromosome (2,837,379 bp), a linear chromosome (2,043,296 bp), and three plasmids (AT1, AT2, and Ti virulence plasmids).
- The Ti virulence plasmid was identified, suggesting its role in pathogenicity.
Conclusions:
- The genome sequence provides a comprehensive genetic resource for Agrobacterium fabrum strain 1D1416.
- The presence of a Ti virulence plasmid in this nondisarmed strain correlates with its ability to induce gall-like structures in citrus tissue, highlighting its pathogenic potential.
