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Partially coherent phase imaging with simultaneous source recovery.

Zhong Jingshan1, Lei Tian2, Justin Dauwels3

  • 1Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore ; Department of Electrical Engineering and Computer Sciences, University of California, Berkeley USA.

Biomedical Optics Express
|February 7, 2015
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Summary
This summary is machine-generated.

We developed a new phase retrieval method using partially coherent light from any source shape. This technique accurately reconstructs sample phase and amplitude while estimating illumination coherence, applicable across various imaging systems.

Keywords:
(100.5070) Phase retrieval(110.3010) Image reconstruction techniques

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Area of Science:

  • Coherent imaging
  • Phase contrast microscopy
  • Image reconstruction

Background:

  • Phase retrieval is crucial for imaging systems using partially coherent light.
  • Existing methods often struggle with arbitrary source shapes and unknown illumination coherence.
  • Köhler geometry is widely used for controlling illumination in microscopy.

Purpose of the Study:

  • To develop a novel phase retrieval method for partially coherent illumination from arbitrary source shapes.
  • To simultaneously recover sample phase/amplitude and estimate illumination spatial coherence.
  • To provide a robust, fast, and experimentally simple solution for phase imaging.

Main Methods:

  • Utilizing a stack of defocused intensity images.
  • Employing a Kalman filtering approach for phase retrieval and source shape estimation.
  • Implementing the method in Köhler geometry with arbitrary source shapes.

Main Results:

  • Successful simultaneous recovery of sample phase and amplitude.
  • Accurate estimation of the unknown source shape, characterizing illumination coherence.
  • Demonstrated robustness to noise and experimental flexibility.

Conclusions:

  • The proposed method offers a versatile and efficient solution for phase retrieval in partially coherent imaging.
  • Applicable to optical, electron, and X-ray microscopy, enhancing imaging capabilities.
  • Experimental validation in an optical microscope confirms the method's practical utility.