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Updated: Apr 17, 2026
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
Published on: March 31, 2019
Metal-ion-mediated assemblies of thiazole orange with cucurbit[7]uril: a photophysical study
Meenakshi N Shinde1, Sharmistha Dutta Choudhury, Nilotpal Barooah
1Radiation & Photochemistry Division, Bhabha Atomic Research Centre , Mod. Lab., Mumbai 400085, Maharashtra, India.
Abstract:
The formation of molecular superstructures by metal-ion-mediated noncovalent self-assembly has been demonstrated using the macrocycle, cucurbit[7]uril (CB7), and the dye, thiazole orange (TO), as building blocks. Interestingly, the association of these molecular building blocks can be tuned by the chemical environment, leading to self-assembled structures of different stoichiometries, which is supported by absorption, fluorescence, (1)H NMR, and AFM measurements. Most importantly, the self-assembly process of the CB7/TO/metal ion system is observed to be remarkably different for alkali (Na(+)) and alkaline earth (Ca(2+)) metal ions. Fluorescence enhancement is observed in the presence of Ca(2+) ions, which is attributed to the formation of short dimeric structures composed of two 1:1 CB7-TO complexes. Solution turbidity is detected in the presence of Na(+) ions, which is proposed to be due to the formation of extended structures by the assembly of many 1:1 CB7-TO complexes.
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