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Updated: Apr 17, 2026

Analysis and Specification of Starch Granule Size Distributions
Published on: March 4, 2021
Changes in crystal structure of chickpea starch samples during processing treatments: an X-ray diffraction and starch
Yongkang Sun1, Hong Ye2, Bing Hu2
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; College of Food and Drug, Anhui Science and Technology University, Fengyang 233100, China.
Two methods accurately measured chickpea starch
Area of Science:
- Food Science
- Materials Science
- Biochemistry
Background:
- Chickpea starch exhibits complex crystalline structures.
- Understanding structural changes during processing is crucial for food applications.
- Differential susceptibility of crystalline forms to enzymatic degradation requires investigation.
Purpose of the Study:
- To compare two methods for determining relative crystallinity (RC) in chickpea starch.
- To investigate the differential susceptibility of A- and B-type polymorphs to α-amylase hydrolysis.
Main Methods:
- X-ray diffraction (XRD) deconvolution method for relative crystallinity (RCA and RCB).
- Moisture analysis method for relative crystallinity (RCA and RCB).
- α-amylase hydrolysis assay to assess enzymatic resistance.
Main Results:
- XRD deconvolution and moisture analysis methods yielded comparable RCA values for chickpea starch.
- Both methods demonstrated similar trends in RCA changes.
- Chickpea starch's crystalline regions were more resistant to α-amylase than amorphous regions.
- B-type polymorph showed greater resistance to α-amylase hydrolysis than A-type polymorph.
Conclusions:
- The XRD deconvolution and moisture analysis methods are reliable for estimating RCA in chickpea starch.
- These methods provide mutual corroboration for structural analysis.
- Chickpea starch's crystalline structure and B-type polymorph offer enhanced resistance to α-amylase degradation.
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