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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
MicroRNA-19a promotes glioma cell growth by repressing LRIG1
Ling-Min Shao1, Ji-An Yang1, Yue-Fei Wang1
1Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
Abstract:
Growing evidence indicates that deregulation of miRNAs contributes to the development of glioma. In present study, we found that the level of miRNA-19a was significantly elevated in glioma tissues and cell lines. Moreover, down-regulation of miRNA-19a dramatically repressed glioma cell growth in vitro and in vivo. Meanwhile, the expression of LRIG1, a tumor suppressor in glioma, was increased following miRNA-19a knockdown. Furthermore, luciferase reporter assay confirmed that LRIG1 was a direct target of miRNA-19a. In addition, silencing of LRIG1 could reverse the suppressive effect of miRNA-19a inhibitor. Taken together, our results demonstrated that down-regulation of miRNA-19a could suppress the growth of glioma cells, at least in part, through up-regulating LRIG1.
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