Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

7.8K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
7.8K
Angle Closure Glaucoma: Treatment01:28

Angle Closure Glaucoma: Treatment

1.7K
Angle-closure glaucoma, or closed-angle glaucoma, is an eye condition where the iris bulges out and blocks the iridocorneal angle, resulting in a buildup of aqueous humor and increased intraocular pressure. Immediate medical attention is necessary due to the sudden onset of symptoms. The treatment for angle-closure glaucoma includes short-term and long-term approaches. Short-term treatment involves using eye drops like pilocarpine to lower intraocular pressure by increasing aqueous humor...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histidine Supplementation Stabilizes Hearing and Vision and Improves Growth in HARS1-Related Autosomal Recessive Disorder Associated With Usher-Like Symptoms.

American journal of medical genetics. Part A·2026
Same author

Longitudinal investigation of prostate tumor spheroid proliferation with dynamic line-field optical coherence tomography.

Biomedical optics express·2026
Same author

Evaluation of GPT-4o and Gemini Advanced on the Korean National Dental Licensing Examination: Accuracy, consistency, and question generation.

Journal of dental sciences·2026
Same author

Imaging of Tissue and Cell Dynamics: introduction to the feature issue.

Biomedical optics express·2026
Same author

Skel-Net: automatic prediction of skeletal pattern on scanned lateral cephalograms using anatomical prior-guided deep learning network.

BMC oral health·2025
Same author

Visual stimulus-evoked transient blood flow and blood vessel diameter changes in the healthy human retina measured with a combined OCT+ERG system.

Biomedical optics express·2025

Related Experiment Video

Updated: Apr 17, 2026

In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation
08:13

In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation

Published on: November 11, 2025

798

Optical edge effects create conjunctival indentation thickness artefacts.

Luigina Sorbara1, Trefford L Simpson, Jyotsna Maram

  • 1School of Optometry & Vision Science, University of Waterloo, Waterloo, Canada; Centre for Contact Lens Research, University of Waterloo, Waterloo, Canada.

Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)
|February 10, 2015
PubMed
Summary
This summary is machine-generated.

An optical artifact, not tissue compression, causes apparent conjunctival indentation in ultrahigh resolution optical coherence tomography (UHR-OCT) images of contact lenses. This artifact was confirmed by imaging lenses on non-biological surfaces.

Keywords:
biomedical imagingconjunctivacontact lens edge profilecorneal imagingophthalmic imagingultra-high resolution optical coherence tomography

More Related Videos

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

2.1K
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

4.5K

Related Experiment Videos

Last Updated: Apr 17, 2026

In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation
08:13

In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation

Published on: November 11, 2025

798
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

2.1K
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

4.5K

Area of Science:

  • Ophthalmology
  • Biomedical Optics
  • Contact Lens Technology

Background:

  • Ultrahigh resolution optical coherence tomography (UHR-OCT) can reveal apparent conjunctival compression at contact lens edges.
  • The origin of this compression is debated, potentially being actual tissue alteration or an optical artifact due to refractive index differences.

Purpose of the Study:

  • To investigate the origins of conjunctival compression observed with contact lenses in UHR-OCT images.
  • To differentiate between actual tissue alteration and optical artifacts.

Main Methods:

  • Contact lenses (silicone hydrogel and hydrogel) were imaged using UHR-OCT on non-biological surfaces (glass sphere, rigid contact lens) and human conjunctiva.
  • Image analysis was performed using ImageJ to quantify observed displacement.

Main Results:

  • Significant optical displacement was observed when imaging lenses on rigid surfaces, confirming an artifact.
  • Displacement values ranged from 5.39 to 11.99 μm on a glass sphere and 5.51 to 9.72 μm on a rigid contact lens.
  • Similar displacement was noted on human conjunctiva, ranging from 6.49 to 17.4 μm.

Conclusions:

  • An optical displacement artifact is a significant factor in UHR-OCT imaging of contact lens edges on rigid surfaces.
  • The displacement observed in vivo likely results from a combination of this optical artifact and actual conjunctival compression.