Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Apr 17, 2026

Functional Calcium Imaging in Developing Cortical Networks

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

Imaging approaches to measuring lysosomal calcium.

Anthony J Morgan¹, Lianne C Davis¹, Antony Galione¹

¹Department of Pharmacology, University of Oxford, Oxford, UK.

Methods in Cell Biology

|February 11, 2015

Summary

Endolysosomes act as calcium (Ca2+) stores, releasing it to regulate cell functions. Monitoring these calcium signals is crucial for understanding endolysosome activity and associated diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Endosomal TPC1-mediated Ca<sup>2+</sup> nanodomains regulate transferrin receptor trafficking and iron homeostasis.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

O-linked glycan-dependent gating of TPC2 controls lysosomal excitability and organelle remodeling.

Nature communications·2026

Same author

A new probe illuminates endo-lysosomal Ca2+ measurements: A role for vesicular IP3 receptors?

The Journal of cell biology·2025

Same author

TRPML2 channel modulation by PI(3,5)P₂ and small-molecule agonists controls endosomal vesicle dynamics.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025

Same author

Dysregulation of the NLRP3 Inflammasome and Promotion of Disease by IL-1β in a Murine Model of Sandhoff Disease.

Cells·2025

Same author

TPC2 gating: Trying to break the enigma code.

Cell calcium·2024

Area of Science:

Cell Biology
Organelle Signaling
Calcium Homeostasis

Background:

Endolysosomes are recognized for their role in intracellular calcium (Ca2+) signaling.
Acidic organelles, including endolysosomes, actively sequester and release Ca2+.
Dysregulation of endolysosomal Ca2+ is linked to various diseases.

Purpose of the Study:

To explore the function of endolysosomes as intracellular Ca2+ stores.
To discuss methods for monitoring endolysosomal Ca2+ signals.
To highlight potential challenges in studying endolysosomal Ca2+.

Main Methods:

Utilizing complementary approaches to measure Ca2+ within endolysosomes.
Investigating Ca2+ flux through endolysosomal channels.
Analyzing the impact of Ca2+ on endolysosomal function.

Keywords:

Bafilomycin A1 Ca(2+)GPN Lysosome NAADP Nigericin pH

More Related Videos

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Published on: March 24, 2014

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Related Experiment Videos

Last Updated: Apr 17, 2026

Functional Calcium Imaging in Developing Cortical Networks

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Published on: March 24, 2014

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Main Results:

Endolysosomes actively sequester Ca2+ in their lumen.
Activation of endolysosomal Ca2+ channels releases Ca2+ to the cytosol.
Local Ca2+ signaling is vital for endolysosomal and global cellular processes.

Conclusions:

Endolysosomes are critical regulators of cellular Ca2+ dynamics.
Accurate monitoring of endolysosomal Ca2+ is essential for disease research.
Understanding Ca2+ handling by endolysosomes offers therapeutic insights.