Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

4.0K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
4.0K
Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellulose Acetate Membrane Electrophoresis Based Urinary Proteomics for the Identification of Characteristic Proteins.

Journal of clinical laboratory analysis·2015
Same author

[Comprehensive analysis of urinary proteins for identification of renal disease markers].

Rinsho byori. The Japanese journal of clinical pathology·2015
Same author

Redox state of urinary albumin in patients with IgA nephropathy.

Rinsho byori. The Japanese journal of clinical pathology·2012
Same author

Benzene risk estimation using radiation equivalent coefficients.

Risk analysis : an official publication of the Society for Risk Analysis·2009
Same author

Molecular heterogeneity of urinary albumin in glomerulonephritis: comparison of cardiovascular disease with albuminuria.

Clinica chimica acta; international journal of clinical chemistry·2009
Same author

Development of a physiologically based pharmacokinetic model for bisphenol A in pregnant mice.

Toxicology and applied pharmacology·2007

Related Experiment Video

Updated: Apr 17, 2026

A Modified Precipitation Method to Isolate Urinary Exosomes
05:05

A Modified Precipitation Method to Isolate Urinary Exosomes

Published on: January 16, 2015

18.2K

[Proteomic analysis of urinary exosomes].

Aki Nakayama

    Rinsho Byori. the Japanese Journal of Clinical Pathology
    |February 12, 2015
    PubMed
    Summary

    Urinary exosomes, small vesicles in urine, are key for cell communication and hold potential as biomarkers for kidney health and disease. Their isolation and analysis are crucial for understanding these roles.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Biomarker Discovery

    Background:

    • Exosomes are nanoscale extracellular vesicles involved in intercellular communication.
    • They are found in various bodily fluids, including urine.
    • Urinary exosomes contain proteins and nucleic acids relevant to physiological and pathophysiological states.

    Purpose of the Study:

    • To provide an overview of urinary exosomes.
    • To discuss methods for isolating urinary exosomes and associated challenges.
    • To highlight the role of urinary exosome proteins as biomarkers for kidney function and disease.

    Main Methods:

    • Review of existing literature on exosome isolation techniques.
    • Analysis of studies identifying protein biomarkers in urinary exosomes.

    More Related Videos

    Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
    14:48

    Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

    Published on: May 8, 2021

    7.8K
    Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
    09:28

    Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

    Published on: October 27, 2023

    3.9K

    Related Experiment Videos

    Last Updated: Apr 17, 2026

    A Modified Precipitation Method to Isolate Urinary Exosomes
    05:05

    A Modified Precipitation Method to Isolate Urinary Exosomes

    Published on: January 16, 2015

    18.2K
    Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
    14:48

    Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

    Published on: May 8, 2021

    7.8K
    Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
    09:28

    Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

    Published on: October 27, 2023

    3.9K
  • Focus on the diagnostic and prognostic potential of urinary exosome content.

    • Main Results:

    • Urinary exosomes are valuable sources of biomarkers for kidney-related conditions.
    • Protein components of urinary exosomes reflect physiological and disease states.
    • Challenges in isolation methods impact biomarker reliability.

    Conclusions:

    • Urinary exosomes represent a promising, non-invasive source for biomarker discovery.
    • Further refinement of isolation techniques is needed for clinical application.
    • Urinary exosome profiling can aid in diagnosing and monitoring kidney diseases.