Related Experiment Video
Updated: Apr 17, 2026
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
A multiple amplification strategy for nucleic acid detection based on host-guest interaction between the
Xiaochen Guo1, Pei Liu, Xiaohai Yang
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. kmwang@hnu.edu.cn yangxiaohai@hnu.edu.cn.
A novel nucleic acid detection method uses a multiple amplification strategy with host-guest interactions for enhanced sensitivity. This sensitive assay accurately quantifies DNA and microRNA in biological samples.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- Nucleic acid detection is crucial for biomedical research and diagnostics.
- Existing methods often face limitations in sensitivity and specificity.
- Developing novel amplification strategies is essential for improved detection.
Purpose of the Study:
- To develop a novel multiple amplification strategy for sensitive nucleic acid detection.
- To utilize host-guest interactions for fluorescence enhancement in the detection system.
- To validate the platform's efficacy in biological samples like human serum.
Main Methods:
- A three-part amplification system combining isothermal strand displacement amplification (SDA) and cyclic enzymatic amplification (CEA).
- Incorporation of host-guest interaction between β-cyclodextrin polymer (β-CDP) and pyrene for fluorescence signal enhancement.
- Detection of target DNA and microRNA (miRNA) using the developed platform.
Main Results:
- Demonstrated a positive linear correlation between target DNA concentration (75 fM to 1 pM) and signal output.
- Achieved a low detection limit of 41 fM for target DNA.
- Successfully validated the assay by detecting miRNA-21 in human serum samples.
Conclusions:
- The developed multiple amplification strategy offers a highly sensitive and specific method for nucleic acid quantification.
- The assay's ability to function in human serum highlights its potential for clinical applications.
- This platform holds significant promise for applications in biomedical research, clinical analysis, and disease diagnostics.
Related Concept Videos
02:57Real Time RT-PCR
The real-time quantification of the number of amplified products is...
03:31Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...