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Carbon nanotube-based separation columns for microchip electrochromatography
K B Mogensen1, B Delacourt, J P Kutter
1Department of Micro and Nanotechnology, Technical University of Denmark, DTU, Building 345 East, 2800, Kongens Lyngby, Denmark.
Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2015
Summary
We developed a new method for creating microchip chromatography devices using carbon nanotube stationary phases. This approach simplifies fabrication by integrating the stationary phase before chip assembly, reducing time and cost.
Area of Science:
- Analytical Chemistry
- Materials Science
- Chemical Engineering
Background:
- Microchip chromatography fabrication is typically complex and labor-intensive.
- Conventional methods involve packing stationary phases post-chip fabrication, leading to inefficiencies.
- Existing protocols are time-consuming and costly, hindering widespread adoption.
Purpose of the Study:
- To present a streamlined protocol for fabricating microchip chromatography devices.
- To detail the use of microfabricated carbon nanotube stationary phases.
- To overcome the challenges associated with conventional stationary phase integration.
Main Methods:
- Utilized lithography to define the carbon nanotube stationary phase within the microchannel.
- Integrated the stationary phase before the chip's lid was bonded.
- Developed detailed fabrication and operation protocols for the new device architecture.
Main Results:
- Successfully fabricated microfluidic devices with integrated carbon nanotube stationary phases.
- Demonstrated a simplified fabrication process that avoids difficult post-fabrication packing.
- Established protocols for the operation of these novel chromatography devices.
Conclusions:
- The described method offers a more efficient and cost-effective approach to microchip chromatography fabrication.
- Microfabricated carbon nanotube stationary phases integrated before bonding simplify device construction.
- This protocol facilitates the development of advanced microfluidic analytical devices.
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