Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

3.1K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.1K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.8K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
2.8K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.8K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.8K
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

3.4K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
3.4K
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

4.4K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time direct cell concentration and viability determination using a fully automated microfluidic platform for standalone process monitoring.

The Analyst·2015
Same author

Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip.

The Analyst·2013
Same author

Solvent-programmed microchip open-channel electrochromatography.

Analytical chemistry·2011
Same author

Validation of a fully autonomous phosphate analyser based on a microfluidic lab-on-a-chip.

Water science and technology : a journal of the International Association on Water Pollution Research·2010
Same author

Carbon nanotubes integrated in electrically insulated channels for lab-on-a-chip applications.

Nanotechnology·2009
Same author

Photonic crystal resonator integrated in a microfluidic system.

Optics letters·2008

Related Experiment Video

Updated: Apr 17, 2026

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K

Carbon nanotube-based separation columns for microchip electrochromatography.

K B Mogensen1, B Delacourt, J P Kutter

  • 1Department of Micro and Nanotechnology, Technical University of Denmark, DTU, Building 345 East, 2800, Kongens Lyngby, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2015
PubMed
Summary

We developed a new method for creating microchip chromatography devices using carbon nanotube stationary phases. This approach simplifies fabrication by integrating the stationary phase before chip assembly, reducing time and cost.

More Related Videos

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.4K
Fabrication of the Thermoplastic Microfluidic Channels
16:00

Fabrication of the Thermoplastic Microfluidic Channels

Published on: February 3, 2008

13.9K

Related Experiment Videos

Last Updated: Apr 17, 2026

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.4K
Fabrication of the Thermoplastic Microfluidic Channels
16:00

Fabrication of the Thermoplastic Microfluidic Channels

Published on: February 3, 2008

13.9K

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Microchip chromatography fabrication is typically complex and labor-intensive.
  • Conventional methods involve packing stationary phases post-chip fabrication, leading to inefficiencies.
  • Existing protocols are time-consuming and costly, hindering widespread adoption.

Purpose of the Study:

  • To present a streamlined protocol for fabricating microchip chromatography devices.
  • To detail the use of microfabricated carbon nanotube stationary phases.
  • To overcome the challenges associated with conventional stationary phase integration.

Main Methods:

  • Utilized lithography to define the carbon nanotube stationary phase within the microchannel.
  • Integrated the stationary phase before the chip's lid was bonded.
  • Developed detailed fabrication and operation protocols for the new device architecture.

Main Results:

  • Successfully fabricated microfluidic devices with integrated carbon nanotube stationary phases.
  • Demonstrated a simplified fabrication process that avoids difficult post-fabrication packing.
  • Established protocols for the operation of these novel chromatography devices.

Conclusions:

  • The described method offers a more efficient and cost-effective approach to microchip chromatography fabrication.
  • Microfabricated carbon nanotube stationary phases integrated before bonding simplify device construction.
  • This protocol facilitates the development of advanced microfluidic analytical devices.