Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

37
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
37
Enzyme Inhibition01:30

Enzyme Inhibition

95.4K
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
95.4K
Feedback Inhibition00:46

Feedback Inhibition

59.0K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
59.0K
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

5.7K
5.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

6.3K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.3K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

3.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane ATG8ylation in secretory autophagy.

Autophagy·2026
Same author

Caspase-8 cleavage of p62/SQSTM1 drives TNFR-induced apoptosis.

Molecular cell·2026
Same author

Microautophagy: current understanding of its molecular mechanisms and functions.

Autophagy reports·2026
Same author

Endothelial Cell Autophagy Suppresses Metastasis In Mouse Mammary and Pancreatic Neuroendocrine Tumor Models.

bioRxiv : the preprint server for biology·2026
Same author

Beyond the Secretory Pathway: New Insights Into Protein Release.

Traffic (Copenhagen, Denmark)·2025
Same author

Phospho-proteome profiling in human neurons reveals targets of TBK1 in ALS/FTD-associated autophagy networks.

Cell reports·2025

Related Experiment Video

Updated: Apr 17, 2026

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.6K

Ironing out VPS34 inhibition.

Timothy Marsh, Jayanta Debnath

    Nature Cell Biology
    |February 14, 2015
    PubMed
    Summary

    VPS34 inhibitors are valuable tools for studying autophagy. These compounds help researchers understand how autophagosome formation is regulated.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Autophagy is a fundamental cellular process for degrading and recycling damaged components.
    • Phosphoinositide 3-kinase (PI3K) class III, also known as VPS34, plays a critical role in initiating autophagosome formation.
    • Dysregulation of autophagy is implicated in various diseases, including cancer and neurodegenerative disorders.

    Purpose of the Study:

    • To highlight the development and utility of VPS34 inhibitors.
    • To underscore the importance of these inhibitors in dissecting the molecular mechanisms of autophagy.
    • To provide researchers with tools for investigating autophagic processes.

    Main Methods:

    • Development of specific small molecule inhibitors targeting VPS34.

    More Related Videos

    Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
    09:39

    Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

    Published on: December 20, 2014

    15.9K
    Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
    10:48

    Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

    Published on: May 15, 2014

    12.0K

    Related Experiment Videos

    Last Updated: Apr 17, 2026

    Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
    10:40

    Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

    Published on: December 21, 2019

    26.6K
    Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
    09:39

    Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

    Published on: December 20, 2014

    15.9K
    Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
    10:48

    Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

    Published on: May 15, 2014

    12.0K
  • Utilizing these inhibitors in cellular models to study autophagosome biogenesis.
  • Employing biochemical and imaging techniques to assess the impact of VPS34 inhibition on autophagy.

    • Main Results:

    • Multiple independent research groups have successfully generated potent VPS34 inhibitors.
    • These inhibitors effectively block autophagosome formation, confirming VPS34's essential role.
    • The developed inhibitors serve as crucial chemical probes for dissecting autophagic pathways.

    Conclusions:

    • VPS34 inhibitors represent significant advancements in autophagy research.
    • These tools enable a deeper understanding of the regulation of autophagosome formation.
    • Further application of VPS34 inhibitors will likely accelerate discoveries in autophagy and related diseases.