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Related Experiment Video

Updated: Apr 17, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Hippo signalling directs intestinal fate.

Marie Le Bouteiller, Kim B Jensen

    Nature Cell Biology
    |February 14, 2015
    PubMed
    Summary

    The Hippo signaling pathway

    Area of Science:

    • Cell biology
    • Developmental biology
    • Gastroenterology

    Background:

    • The Hippo signaling pathway regulates organ size and is crucial for tissue homeostasis.
    • YAP (Yes-associated protein) and TAZ (Transcriptional coactivator with PDZ-binding motif), effectors of the Hippo pathway, have proposed distinct roles in the intestinal epithelium.

    Purpose of the Study:

    • To elucidate the specific functions of YAP and TAZ in the intestinal epithelium.
    • To investigate their roles in regulating intestinal stem cell proliferation and differentiation.

    Main Methods:

    • Utilized mouse models and advanced imaging techniques.
    • Employed genetic manipulation to study YAP and TAZ.
    • Analyzed gene expression and cell lineage tracing.

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    Main Results:

    • YAP and TAZ exhibit a dual role in the intestinal epithelium.
    • They are essential for regulating both stem cell proliferation and differentiation.
    • Their activity directs differentiation towards a specific secretory cell lineage.

    Conclusions:

    • YAP and TAZ are key regulators of intestinal homeostasis.
    • They coordinate stem cell behavior to ensure proper epithelial renewal and function.
    • Understanding their dual role provides insights into intestinal development and disease.