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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Published on: November 1, 2017
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Retinoic acid regulates hematopoietic development from human pluripotent stem cells.
Roger E Rönn1, Carolina Guibentif1, Roksana Moraghebi1
1Department of Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden.
Stem Cell Reports
|February 15, 2015
Summary
Decreased retinoic acid (RA) signaling enhances human hematopoietic stem cell (HSC)-like cell generation. This study reveals RA
Area of Science:
- Developmental Biology
- Hematopoiesis
- Stem Cell Research
Background:
- Retinoic acid (RA) is a crucial morphogen in embryogenesis.
- Its specific roles in human developmental hematopoiesis require further investigation.
Purpose of the Study:
- To evaluate the effects of RA signaling on human blood development and hematopoietic progenitors in vitro.
- To elucidate the mechanisms by which RA influences hematopoietic stem cell generation.
Main Methods:
- Utilized an in vitro model of human hematopoietic development.
- Assessed the impact of altered RA signaling on pluripotent stem cell commitment and progenitor differentiation.
Main Results:
- Reduced RA signaling increased the generation of hematopoietic stem cell (HSC)-like cells.
- This increase occurred via enhanced commitment of pluripotent stem cells to hematopoiesis and decreased differentiation of blood progenitors.
- Identified two distinct mechanisms influenced by RA signaling levels.
Conclusions:
- Controlled, low-level RA signaling is essential for human blood development.
- RA acts as a regulatory factor, where precise control promotes hematopoietic progenitor cell generation from pluripotent stem cells in vitro.
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