Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

8.4K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
8.4K
Electrophoresis: Overview01:20

Electrophoresis: Overview

5.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
5.2K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.8K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.8K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

122.4K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
122.4K
SDS-PAGE01:27

SDS-PAGE

36.8K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
36.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct molecular epidemiology and outcomes of carbapenem-resistant gram-negative infections in Lebanon: insights from the MDRO network.

BMC infectious diseases·2026
Same author

Loss of O-antigen due to <i>wbbL</i> mutations is common and associated with increased mortality in <i>Escherichia coli</i> bloodstream infections.

Science advances·2026
Same author

Rationalising heterogeneity in Staphylococcus aureus bacteraemia: current progress and future goals.

The Journal of infectious diseases·2026
Same author

Development and Application of a Desirability of Outcome Ranking (DOOR) Endpoint for Acute Bacterial Skin and Skin Structure Infections.

Open forum infectious diseases·2026
Same author

Timing of Bronchoscopy and Plasma Microbial Cell-Free DNA Sequencing in Immunocompromised Host Pneumonia.

Open forum infectious diseases·2026
Same author

Treating penicillin-susceptible Staphylococcus aureus bacteraemia: confusing the issue with facts.

Lancet (London, England)·2026

Related Experiment Video

Updated: Apr 17, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

798.5K

Pulse Field Gel Electrophoresis.

Batu K Sharma-Kuinkel1, Thomas H Rude1, Vance G Fowler2

  • 1Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2015
PubMed
Summary

Pulse Field Gel Electrophoresis (PFGE) separates large DNA molecules for bacterial genotyping. This advanced technique offers highly reproducible and well-resolved DNA fragment profiles for comprehensive genomic analysis.

Keywords:
Genomic DNAGenotyping techniquePulse field gel electrophoresisRestriction enzyme

More Related Videos

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

19.9K
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

14.2K

Related Experiment Videos

Last Updated: Apr 17, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

798.5K
Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

19.9K
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

14.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Conventional methods struggle with large DNA fragments.
  • Genomic analysis requires high-resolution separation techniques.

Purpose of the Study:

  • To introduce Pulse Field Gel Electrophoresis (PFGE) as a superior genotyping method.
  • To highlight PFGE's capability in analyzing large DNA molecules.

Main Methods:

  • Digesting entire genomic DNA with restriction enzymes.
  • Separating DNA fragments using an electric field with periodically changing directions in a gel matrix.
  • Utilizing a variation of agarose gel electrophoresis.

Main Results:

  • PFGE enables analysis of bacterial DNA fragments significantly larger than conventional methods.
  • The technique provides a comprehensive representation of the bacterial chromosome.
  • PFGE yields highly reproducible restriction profiles with distinct, well-resolved DNA fragments.

Conclusions:

  • PFGE is a powerful and effective technique for bacterial DNA genotyping.
  • Its ability to resolve large DNA fragments offers significant advantages over traditional methods.
  • PFGE provides reliable and detailed genomic profiles for research and diagnostics.