Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Review on the Bioavailability, Bioefficacies, and Delivery Systems for Sanshools, the Main Numbing Substances in <i>Zanthoxylum bungeanum</i>.

Journal of agricultural and food chemistry·2026
Same author

Microglial polarization in retinal neovascularization: Friend or foe?

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2026
Same author

The role and underlying mechanisms of Qi Gong Wan in enhancing the endometrial receptivity of a rat model with polycystic ovary syndrome.

Frontiers in reproductive health·2026
Same author

Factors associated with anticipatory grief in family caregivers of patients with esophageal cancer: a cross-sectional study.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

Exploring the factors influencing anticipatory grief in esophageal cancer patients: A cross-sectional study.

European journal of oncology nursing : the official journal of European Oncology Nursing Society·2025
Same author

Exosomal miRNA Profiling in Liquid Biopsy of Vitreous in Proliferative Diabetic Retinopathy.

Investigative ophthalmology & visual science·2025

Related Experiment Video

Updated: Apr 17, 2026

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

10.6K

pH-responsive deoxyribonucleic acid capture/release by polydopamine functionalized magnetic nanoparticles.

Yu Wang1, Xiangdong Ma1, Chun Ding1

  • 1Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

Analytica Chimica Acta
|February 16, 2015
PubMed
Summary

Researchers developed polydopamine functionalized magnetic nanoparticles (PDA@Fe3O4) for efficient genomic deoxyribonucleic acid (DNA) extraction. This eco-friendly method simplifies DNA purification for subsequent sensitive detection in food safety applications.

Keywords:
BacteriaCapillary electrophoresisDeoxyribonucleic acidMagnetic nanoparticlesPolydopaminePolymerase chain reaction

More Related Videos

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release
08:39

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Published on: July 4, 2017

9.6K
Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
07:58

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

Published on: November 14, 2018

8.9K

Related Experiment Videos

Last Updated: Apr 17, 2026

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

10.6K
Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release
08:39

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Published on: July 4, 2017

9.6K
Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
07:58

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

Published on: November 14, 2018

8.9K

Area of Science:

  • Nanomaterials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Efficient isolation of high-quality deoxyribonucleic acid (DNA) is crucial for molecular diagnostics.
  • Traditional DNA extraction methods often involve toxic reagents and multiple steps.
  • Developing novel, rapid, and environmentally friendly DNA extraction techniques is essential.

Purpose of the Study:

  • To synthesize and characterize polydopamine functionalized magnetic nanoparticles (PDA@Fe3O4).
  • To evaluate the efficiency of PDA@Fe3O4 for genomic DNA extraction via magnetic solid-phase extraction (MSPE).
  • To demonstrate the application of the developed method for simultaneous detection of foodborne pathogens.

Main Methods:

  • Synthesis and characterization of PDA@Fe3O4 nanoparticles using electron microscopy and magnetometry.
  • Optimization of DNA binding and elution using pH-controlled aqueous solutions.
  • Integration of MSPE with polymerase chain reaction (PCR) and capillary electrophoresis (CE) for pathogen detection.

Main Results:

  • PDA@Fe3O4 nanoparticles exhibited high adsorption capacity for genomic DNA (161 mg g(-1)).
  • The pH-switchable binding mechanism enabled efficient DNA extraction without toxic reagents.
  • Extracted DNA was suitable for direct use in PCR and CE analysis.
  • The integrated MSPE-PCR-CE method successfully detected Listeria monocytogenes and Escherichia coli O157:H7 in milk samples.

Conclusions:

  • PDA@Fe3O4 based MSPE offers a superior, green alternative for DNA extraction compared to commercial kits and phenol-chloroform methods.
  • The developed method provides a rapid, sensitive, and efficient platform for simultaneous pathogen detection in food matrices.
  • This approach holds significant potential for improving food safety monitoring and diagnostics.