Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.1K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.1K
RNA Interference01:23

RNA Interference

29.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
29.0K
RNA Interference01:23

RNA Interference

7.9K
7.9K
Types of RNA01:23

Types of RNA

74.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
74.3K
Types of RNA01:20

Types of RNA

16.8K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
16.8K
Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional structure of the palbociclib-HIV TAR complex.

Nucleic acids research·2026
Same author

The kinase inhibitor palbociclib binds to HIV TAR RNA with very low nanomolar affinity and exquisite specificity.

Nucleic acids research·2026
Same author

Structure of Essential RNA Regulatory Elements in the West Nile Virus 3'-Terminal Stem Loop.

Journal of molecular biology·2024
Same author

Small molecules targeting microRNAs: new opportunities and challenges in precision cancer therapy.

Trends in cancer·2024
Same author

Two distinct binding modes provide the RNA-binding protein RbFox with extraordinary sequence specificity.

Nature communications·2023
Same author

Drug-Like Small Molecules That Inhibit Expression of the Oncogenic MicroRNA-21.

ACS chemical biology·2023
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

Related Experiment Video

Updated: Apr 17, 2026

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

8.9K

Structure based approaches for targeting non-coding RNAs with small molecules.

Matthew D Shortridge1, Gabriele Varani1

  • 1Department of Chemistry, University of Washington, Seattle, Box 351700, Seattle 98195, USA.

Current Opinion in Structural Biology
|February 18, 2015
PubMed
Summary
This summary is machine-generated.

Small molecules offer a promising alternative to oligonucleotide therapies for targeting non-coding RNAs (ncRNAs) involved in human diseases. This review explores new chemical strategies for developing ncRNA-targeted small molecule drugs.

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.6K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.3K

Related Experiment Videos

Last Updated: Apr 17, 2026

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

8.9K
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.6K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), play crucial roles in chronic and degenerative diseases.
  • RNA targets are validated by existing antibacterial and antiviral therapies, highlighting their therapeutic potential.
  • Oligonucleotide-based therapies face significant pharmacological challenges, necessitating alternative approaches.

Purpose of the Study:

  • To review recent advancements in small molecule-based strategies for targeting ncRNAs.
  • To explore novel structural and chemical principles for developing small molecule RNA therapeutics.
  • To present small molecules as a viable alternative to oligonucleotide-based approaches for ncRNA-targeted therapies.

Main Methods:

  • Literature review of recent studies on small molecule-RNA interactions.
  • Analysis of structural and chemical properties enabling small molecule targeting of ncRNAs.
  • Discussion of the advantages and limitations of small molecule approaches compared to oligonucleotides.

Main Results:

  • Small molecules represent a promising alternative to oligonucleotide-based therapies for ncRNA targeting.
  • Emerging structural and chemical principles are guiding the development of novel small molecule RNA-targeting agents.
  • Recent research demonstrates the feasibility of modulating ncRNA function with small molecules.

Conclusions:

  • Small molecule-based targeting of ncRNAs presents a viable therapeutic strategy for various human diseases.
  • Further research into structural and chemical principles will accelerate the development of effective small molecule RNA therapeutics.
  • This approach holds potential to overcome limitations associated with oligonucleotide-based therapies.