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High-throughput and label-free single nanoparticle sizing based on time-resolved on-chip microscopy.

Euan McLeod1,2, T Umut Dincer1,2, Muhammed Veli1,2

  • 1†Electrical Engineering Department, University of California, Los Angeles, California 90095, United States.

ACS Nano
|February 18, 2015
PubMed
Summary

A new, cost-effective hand-held device uses holographic microscopy and nanolens assembly for high-throughput nanoparticle sizing. This portable platform enables accurate, label-free detection of particles from 40 nm to millimeter scale in diverse settings.

Keywords:
field-portablelensfree microscopynanoparticlesparticle-sizing

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Area of Science:

  • Nanotechnology
  • Microscopy
  • Analytical Chemistry

Background:

  • Accurate nanoparticle sizing is crucial for nanomaterial synthesis, environmental monitoring, and medical diagnostics.
  • Existing methods often lack the high-throughput, cost-effectiveness, and portability required for field applications.
  • There is a need for accessible, low-resource nanoparticle sizing solutions.

Purpose of the Study:

  • To develop a high-throughput, cost-effective, and portable nanoparticle sizing platform.
  • To enable label-free detection and sizing of individual nanoparticles and complex mixtures.
  • To provide an accessible tool for researchers in low-resource settings and citizen scientists.

Main Methods:

  • Combines holographic on-chip microscopy with vapor-condensed nanolens self-assembly.
  • Utilizes a cost-effective, hand-held device for in situ measurements.
  • Captures time-resolved images for signal enhancement and reliable sizing.

Main Results:

  • Achieved label-free detection and sizing of individual subwavelength particles ( < λ/10) with ±11 nm accuracy.
  • Demonstrated sizing of diverse samples, including viruses and polydisperse mixtures, across 5 orders-of-magnitude in concentration.
  • Enabled measurement of particle sizes ranging from 40 nm to millimeter scale over a 30 mm² field-of-view.

Conclusions:

  • The developed platform offers a significant advancement in accessible nanoparticle characterization.
  • Its portability and cost-effectiveness make advanced nanoscopic measurements available globally.
  • Potential applications span environmental monitoring, biomedical diagnostics, education, and citizen science initiatives.