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A SERS-Assisted 3D Barcode Chip for High-Throughput Biosensing.

Lei Wu1, Zhuyuan Wang1, Kequan Fan1

  • 1Advanced Photonics Center, Southeast University, Nanjing, 210096, China.

Small (Weinheim an Der Bergstrasse, Germany)
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A novel surface-enhanced Raman scattering (SERS)-assisted 3D barcode chip enables high-throughput biosensing. This technology achieves ultrasensitive, multiplexed detection of proteins in under 30 minutes for biomedical applications.

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3D barcodes, barcodesbiosensingmicrofluidic chipssurface enhanced Raman scattering

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • High-throughput biosensing is crucial for disease diagnosis and drug discovery.
  • Existing methods often face limitations in sensitivity, multiplexing capability, or speed.
  • Surface-enhanced Raman scattering (SERS) offers high sensitivity and unique spectral fingerprints.

Purpose of the Study:

  • To develop a novel SERS-assisted 3D barcode chip for high-throughput multiplexed biosensing.
  • To demonstrate the chip's capability in simultaneous detection of multiple protein targets.
  • To achieve ultrasensitive and rapid quantification of analytes.

Main Methods:

  • Development of a 3D barcode chip combining 2D spatial encoding with SERS spectroscopic encoding.
  • Utilizing a microfluidic platform for spatial separation of samples and targets via antibody-patterned substrates.
  • Employing SERS probes as "SERS tags" for spectroscopic identification and quantification.

Main Results:

  • Successful demonstration of multiplex immunoassay for simultaneous detection of multiple proteins.
  • Achieved one-step multiplex detection within 30 minutes.
  • Reached an ultrasensitive detection limit of 10 fg mL(-1) (approximately 70 aM).

Conclusions:

  • The SERS-assisted 3D barcode chip provides a powerful platform for high-throughput biomedical applications.
  • The integrated spatial and spectroscopic encoding enables efficient and sensitive multiplexed detection.
  • This technology holds promise for advancing rapid diagnostics and personalized medicine.