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Poxviral ankyrin proteins.

Michael H Herbert1, Christopher J Squire2, Andrew A Mercer3

  • 1School of Biological Sciences, University of Auckland, Auckland 1010, New Zealand. mher065@aucklanduni.ac.nz.

Viruses
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Summary
This summary is machine-generated.

Poxviruses uniquely possess ankyrin repeat (ANK) proteins, likely inherited from an ancient ancestor. These viral proteins interact with cellular machinery, potentially manipulating host responses and viral host range.

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Area of Science:

  • Virology
  • Molecular Biology
  • Protein Science

Background:

  • Ankyrin (ANK) motifs are common in cellular proteins but rare in viruses.
  • Poxviruses, particularly chordopoxviruses, are a notable exception, encoding numerous ANK repeat proteins.

Purpose of the Study:

  • To investigate the evolutionary origin and functional significance of ANK repeat proteins in chordopoxviruses.
  • To understand the molecular mechanisms by which these viral proteins interact with host cell pathways.

Main Methods:

  • Phylogenetic analysis of poxviral ANK repeat protein orthologues.
  • Structural analysis of ANK repeat protein topology and F-box domain.
  • Identification of protein interaction partners and functional assays.

Main Results:

  • ANK repeat proteins were present in an ancestral vertebrate poxvirus and have expanded through duplication.
  • Poxviral ANK proteins feature an N-terminal ANK array and a C-terminal F-box for SCF ubiquitin ligase interaction.
  • These proteins interact with host targets, including the NF-κB antiviral pathway, and influence viral host range.

Conclusions:

  • Poxviral ANK repeat proteins are ancient viral components that have evolved diverse substrate interactions.
  • The F-box motif facilitates manipulation of host ubiquitination pathways for viral benefit.
  • These proteins play a significant role in poxvirus pathogenesis and host interaction.