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Updated: Apr 17, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
Published on: August 2, 2018
Coupling a solid phase microextraction (SPME) probe with ambient MS for rapid enrichment and detection of
Yaoyao Zhao1, Xiaoyun Gong, Xingyu Si
1Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 10084, China. xrzhang@mail.tsinghua.edu.cn.
Abstract:
In this study, we developed a probe-electrospray ionization method by coupling a SPME probe modified with nanosized TiO2 directly to nanoESI-MS for the phosphoproteome analysis, which demonstrated excellent selectivity and sensitivity for enrichment of phosphopeptides in complex biological samples.
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