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Updated: Apr 17, 2026

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine PEI

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine PEI

Published on: May 24, 2022

A novel reduction-sensitive modified polyethylenimine oligonucleotide vector.

Shuang Yang¹, Robert J Lee², Xuewei Yang¹

¹School of Life Sciences, Jinlin University, Changchun 130012, China.

International Journal of Pharmaceutics

|February 21, 2015

Summary

This summary is machine-generated.

A novel reduction-sensitive polyethylenimine derivative (PEI-SS-OA) enhances oligonucleotide delivery and reduces cytotoxicity compared to standard PEI. This makes it a promising candidate for in vivo applications.

Keywords:

Disulfide Oligonucleotide delivery Polyethylenimine Reduction-sensitive

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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine PEI

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Published on: March 29, 2019

Area of Science:

Biomaterials Science
Gene Delivery Systems
Polymer Chemistry

Background:

Polyethylenimine (PEI) is widely used for gene delivery but suffers from high cytotoxicity.
Developing safer and more efficient PEI derivatives is crucial for therapeutic applications.

Purpose of the Study:

To synthesize and evaluate a reduction-sensitive cross-linked PEI derivative, PEI-SS-OA, for enhanced oligonucleotide delivery.
To compare the efficacy and cytotoxicity of PEI-SS-OA with conventional PEI (25 kDa).

Main Methods:

Synthesis of PEI-SS-OA, a reduction-sensitive cross-linked polyethylenimine derivative.
Complexation of PEI-SS-OA with LOR-2501 oligonucleotide.
Confirmation of PEI-SS-OA degradation using dithiothreitol and gel retardation assay.
In vitro evaluation of cytotoxicity and oligonucleotide delivery efficiency.

Main Results:

PEI-SS-OA successfully condensed the LOR-2501 oligonucleotide into positively charged complexes.
Reductive degradation of PEI-SS-OA was confirmed.
PEI-SS-OA demonstrated lower cytotoxicity and higher oligonucleotide delivery efficiency compared to 25 kDa PEI.

Conclusions:

The reduction-sensitive PEI-SS-OA offers improved oligonucleotide delivery efficiency and reduced cytotoxicity.
PEI-SS-OA is a promising candidate for further in vivo evaluation in gene therapy applications.