Related Experiment Video

Updated: Apr 17, 2026

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals
08:22

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

Published on: February 20, 2020

8.1K

[Isotopic effects of low concentration of deuterium in water on biological systems]

A A Kirkina, V I Lobyshev, O D Lopina

    Biofizika
    |February 24, 2015
    PubMed

    Keywords:
    Deuterium effectsIsotopic sensitivityWater concentrationBiological response

    More Related Videos

    Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-109:00

    Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

    Published on: April 16, 2018

    10.9K
    Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization05:55

    Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization

    Published on: June 17, 2025

    993

    Related Concept Videos

    ¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

    ¹H NMR of Labile Protons: Deuterium (²H) Substitution

    This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
    1.5K
    Isotopes and Radioisotopes01:28

    Isotopes and Radioisotopes

    In the early 1900s, English chemist Frederick Soddy realized that an element could have atoms with different masses that were chemically indistinguishable. These different types are called isotopes — atoms of the same element that differ in mass. Isotopes differ in mass because they have different numbers of neutrons but are chemically identical because they have the same number of protons. Soddy was awarded the Nobel Prize in Chemistry in 1921 for this discovery.
    An isotope containing...
    13.9K
    ¹³C NMR: ¹H–¹³C Decoupling01:04

    ¹³C NMR: ¹H–¹³C Decoupling

    The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
    A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
    2.1K
    Mass Spectrometry: Isotope Effect01:13

    Mass Spectrometry: Isotope Effect

    Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
    5.1K
    Chemical Shift: Internal References and Solvent Effects01:17

    Chemical Shift: Internal References and Solvent Effects

    In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
    The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
    1.6K
    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

    When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
    1.9K