Cell surface receptors: rapid quantification of live cell receptors using bioluminescence in a flow-based
Ramesh Ramji1, Cheong Fook Cheong, Hiroaki Hirata
1Department of Biomedical Engineering, 9 Engineering Drive 1, Block EA, #03-12, National University of Singapore, Singapore, 117575.
Small (Weinheim an Der Bergstrasse, Germany)
|February 24, 2015
Abstract:
C. T. Lim and co-workers describe a rapid and sensitive bioluminescence-based microfluidic method for quantifying receptor numbers on live cells. On page 943, this integrated, lens-free optical platform allows the determination of signals from the cell surface with high sensitivity. Compared to conventional approaches, the combined use of bioluminescence and microfluidics makes it safe to use, reduces background noise, improves sensitivity, requires smaller sample volumes, and allows high-throughput sampling over thousands of cells.
