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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Dec 28, 2025

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Peptide-based biosensors.

Qingtao Liu¹, Jinfeng Wang², Ben J Boyd¹

¹Drug Delivery Disposition and Dynamics-Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville 3052, VIC, Australia; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville 3052, VIC, Australia.

|February 24, 2015

Summary

This summary is machine-generated.

Peptide-based biosensors utilize peptides as recognition elements and signal generators for detecting various analytes. Bio-conjugation strategies and advanced techniques enable sensitive and selective detection for applications like clinical diagnosis.

Keywords:

Biosensor Fluorophore Kinase Peptides Protease Protein

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Published on: March 12, 2015

Area of Science:

Biotechnology
Analytical Chemistry
Materials Science

Background:

Peptides are versatile components in biosensor development due to established synthesis, diverse structures, and enzyme substrate capabilities.
Bio-conjugation strategies effectively translate peptide-analyte interactions into measurable signals for biosensor fabrication.
Environmentally sensitive fluorophores, nanoparticles, and other markers enhance signal transduction in peptide-based sensors.

Purpose of the Study:

To review the role of peptides in the design and fabrication of novel biosensors.
To highlight bio-conjugation strategies for signal generation in peptide-based biosensors.
To discuss the application of peptides as bio-recognition elements and enzyme substrates in biosensing.

Main Methods:

Utilizing peptides as bio-recognition elements for diverse analytes (proteins, nucleic acids, bacteria, metal ions, enzymes, antibodies).
Employing peptides as selective enzymatic substrates for enzyme and enzyme-activity sensors.
Conjugating peptides with signal markers such as environmentally sensitive fluorophores, nanoparticles, fluorescent polymers, graphene, and near-infrared dyes.
Leveraging immobilization and microarray techniques for chip-based peptide biosensor development.

Main Results:

Peptides serve as crucial bio-recognition elements, binding to a wide range of analytes.
Peptide selectivity as enzyme substrates enables the construction of specific enzyme sensors.
Integration with various signal transduction components (fluorophores, nanoparticles) leads to sensitive detection.
Advancements in peptide immobilization and microarray technology facilitate chip-based biosensor applications.

Conclusions:

Peptide-based biosensors offer a powerful platform for sensitive and selective analyte detection.
Bio-conjugation and advanced material integration are key to enhancing biosensor performance.
Peptide biosensors show significant potential for commercial applications, particularly in clinical diagnosis.