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Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
Published on: June 10, 2021
Development of imidazoline-2-thiones based two-photon fluorescence probes for imaging hypochlorite generation in a
Qingling Xu1, Cheol Ho Heo, Gyoungmi Kim
1Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750 (Korea).
Abstract:
We designed and prepared the imidazoline-2-thione containing OCl(-) probes, PIS and NIS, which operate through specific reactions with OCl(-) that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl(-) generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl(-) in live cells and tissues, indicating that this probe could have wide biological applications.

