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Updated: Apr 17, 2026

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
Published on: November 18, 2011
Effect of proinflammatory activation on F-actin distribution in cultured human endothelial cells under conditions of
E G Rudimov1, S V Buravkov, E P Andreeva
1Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia, rydimov@gmail.com.
Abstract:
We compared the state of actin cytoskeleton, morphology, and expression of VE-cadherin in endothelial cells of human umbilical cord vein under conditions of TNF-α-mediated activation and microgravity modeling and found that 3D-clinorotation for 24 h impaired the integrity of endothelial monolayer, altered cell morphology, induced cytoskeleton reorganization, and reduced the expression of VE-cadherin. The combination of experimental microgravity and proinflammatory activation led to more pronounced clearing of the perinuclear space from microfilaments and accumulation of depolymerized actin, which confirms additive effect of the studied factors on actin cytoskeleton of endothelial cells.
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