High repetition rate atmospheric pressure matrix-assisted laser desorption/ionization in combination with liquid
Abstract:
One major drawback of matrix-assisted laser desorption/ionization (MALDI) is still the relatively poor pulse-to-pulse reproducibility of the signal intensity. This problem, caused by insufficient homogeneity in the matrix/analyte co-crystallization, is usually circumvented by averaging the detected ion intensity over several shots. However, during the consecutive laser pulses, the applied matrix gets depleted and only a number of subsequent experiments can be done on the same sample spot. In order to achieve the desired long-term stability in combination with a sufficient pulse-to-pulse reproducibility, recently liquid MALDI matrices have been introduced. This contribution demonstrates the promising combination of liquid matrices with high repetition rate lasers for atmospheric pressure MALDI (AP-MALDI). To demonstrate the robustness of the new approach, two different kinds of liquid matrices were used in combination with both a typical flashlamp pumped 15 Hz laser and a diode pumped solid state laser operated at 5 kHz. The latter showed a stable ion signal over more than 3,500,000 consecutive laser pulses.
More Related Videos
10:47Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
Published on: March 24, 2016
06:56Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
Published on: June 10, 2018
Related Concept Videos
Matrix-Assisted Laser Desorption Ionization (MALDI)
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry
