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Related Concept Videos

Golgi Matrix Proteins01:12

Golgi Matrix Proteins

2.6K
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
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Golgi Apparatus01:09

Golgi Apparatus

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Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
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Golgi Apparatus01:49

Golgi Apparatus

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As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
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Golgi Apparatus01:09

Golgi Apparatus

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Coat Assembly and GTPases01:33

Coat Assembly and GTPases

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Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
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Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Related Experiment Video

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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

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How Rab proteins determine Golgi structure.

Shijie Liu1, Brian Storrie1

  • 1Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

International Review of Cell and Molecular Biology
|February 25, 2015
PubMed
Summary
This summary is machine-generated.

Rab proteins regulate Golgi organization. Rab6 depletion alters Golgi structure and vesicle transport, highlighting its role in maintaining Golgi ribbon integrity through motor protein interactions.

Keywords:
Golgi apparatusGolgi ribbon organizationRab41Rab6Rab6 subfamily

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Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Rab proteins, small GTPases, are crucial for mammalian Golgi apparatus organization.
  • Rab proteins are functionally classified into two groups based on their activation state's effect on Golgi structure.

Purpose of the Study:

  • To investigate the role of Rab proteins, particularly Rab6, in mammalian Golgi organization.
  • To understand the mechanisms by which Rab proteins influence Golgi ribbon structure and vesicle transport.

Main Methods:

  • Functional classification of Rab proteins based on Golgi organization assays.
  • Rab6 depletion in HeLa cells to observe effects on Golgi cisternae and vesicle accumulation.
  • Effector studies to identify key Rab6 interacting proteins, including motor proteins.

Main Results:

  • Rab6 depletion leads to increased Golgi cisternal number, longer cisternae, and vesicle accumulation.
  • Rab6's effect on Golgi ribbon organization is likely mediated by regulating vesicle transport.
  • Motor proteins and their regulators are identified as critical Rab6 effectors.

Conclusions:

  • Rab6 plays a significant role in maintaining Golgi ribbon organization, potentially through the regulation of vesicle transport.
  • The balance of motor protein recruitment is a key Rab-dependent factor in Golgi ribbon organization.
  • Rab41 exhibits a contrasting effect on Golgi ribbon organization compared to Rab6.