A New Lignan Glucoside from Lagochilus ilicifolius

Area of Science:

• Phytochemistry
• Pharmacology
• Natural Products Chemistry

Background:

• Lagochilus ilicifolius is a traditional Chinese folk medicine used for hemostasis, inflammation, and ulcers.
• Limited research exists on its chemical constituents and pharmacological effects.

Purpose of the Study:

• To isolate and identify compounds from L. ilicifolius.
• To evaluate the cytotoxic activity of isolated compounds.

Main Methods:

• Column chromatography was employed to separate compounds from the n-butanol-soluble fraction of L. ilicifolius.
• Spectroscopic analyses were used for structure elucidation.
• Cytotoxicity was assessed using the MTT assay against PC12 cell line.

Main Results:

• One novel lignan, erythro-1-[(4-O-β-D-glucopyranosyl-3-methoxyl)-phenyl]-2-[(5'-methoxyl)-pinoresinol]-propane-1,3-diol, was identified.
• Two known lignans, tortoside C and sisymbrifolin, were also isolated.
• The new lignan displayed significant cytotoxic activity (IC50 = 1.22 ± 0.03 μmol/L) against PC12 cells.

Conclusions:

• A new lignan and two known lignans were successfully isolated from L. ilicifolius.
• Tortoside C and sisymbrifolin represent the first reported lignans from the genus Lagochilus.
• The novel lignan exhibits potent in vitro cytotoxicity, while the known lignans showed no significant activity.