Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Screening for multiple endocrine neoplasia type 2a with DNA-polymorphism analysis.

H Sobol1, S A Narod, Y Nakamura

  • 1Unit of Mechanisms of Carcinogenesis, International Agency for Research on Cancer, Lyon, France.

The New England Journal of Medicine
|October 12, 1989
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety and glycemic outcomes of DBLG1 coupled to the DANA-i pump in adults and adolescents with type 1 diabetes mellitus. The SP8 trial.

Journal of endocrinological investigation·2025
Same author

Practical implementation of automated insulin delivery systems in 2025: A French position statement update.

Diabetes & metabolism·2025
Same author

Real-world effectiveness of ixazomib combined with lenalidomide and dexamethasone in relapsed/refractory multiple myeloma: the REMIX study.

Annals of hematology·2023
Same author

Practical implementation of automated closed-loop insulin delivery: A French position statement.

Diabetes & metabolism·2020
Same author

OpenMATB: A Multi-Attribute Task Battery promoting task customization, software extensibility and experiment replicability.

Behavior research methods·2020
Same author

Modeling the variability of insulin sensitivity for people with Type 1 Diabetes based on clinical data from an artificial pancreas study.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020

Genetic screening using DNA probes accurately identifies carriers of the multiple endocrine neoplasia type 2a (MEN2A) gene. This genetic testing is more effective than traditional methods, especially for younger individuals, improving cancer risk prediction.

Area of Science:

  • Human Genetics
  • Oncology
  • Molecular Biology

Background:

  • Multiple Endocrine Neoplasia type 2a (MEN2A) is a hereditary cancer syndrome genetically linked to chromosome 10.
  • Accurate identification of gene carriers is crucial for early detection and management of MEN2A.

Purpose of the Study:

  • To evaluate the utility of DNA probes and restriction-fragment-length polymorphisms (RFLP) for identifying MEN2A gene carriers.
  • To assess the effectiveness of genetic screening compared to conventional endocrine challenge tests for MEN2A.

Main Methods:

  • A genetic linkage study involving 130 individuals from 11 families of European and North African descent.
  • Utilized polymorphic DNA probes to analyze restriction-fragment-length polymorphisms (RFLP) associated with the MEN2A gene.

Related Experiment Videos

  • Compared RFLP analysis with conventional endocrine challenge tests for carrier status prediction.

    • Main Results:

    • No recombination was observed between the MEN2A mutation and two of the three DNA markers used across the families.
    • RFLP analysis proved more effective than endocrine challenge in predicting carrier status, particularly in younger individuals.
    • Genetic counseling was adequately supported by linkage information for 8 out of 11 families.

    Conclusions:

    • Genetic screening via RFLP analysis offers a highly certain method for identifying individuals at risk for MEN2A at any age.
    • Combining DNA screening with endocrine tests maximizes accuracy in carrier status determination.
    • DNA-based screening enables targeted early neoplastic change testing for high-risk individuals.