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Related Experiment Video

Updated: Apr 17, 2026

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
08:55

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

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Whole-mount single molecule FISH method for zebrafish embryo.

Yuma Oka1, Thomas N Sato2

  • 11] Kyoto University, Graduate School of Biostudies, Kyoto, Japan [2] ERATO Sato Live Bio-Forecasting Project, Japan Science and Technology Agency (JST), Kyoto, Japan [3] Advanced Telecommunications Research Institute International (ATR), The Thomas N. Sato BioMEC-X Laboratories, Kyoto, Japan.

Scientific Reports
|February 26, 2015
PubMed
Summary

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This summary is machine-generated.

Researchers developed a new single molecule FISH method for whole-mount zebrafish embryos. This technique allows for quantitative measurement of gene expression noise in individual cells, aiding developmental and disease studies.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Gene expression noise drives cellular adaptability and heterogeneity.
  • Quantitative measurement of gene expression noise is crucial for understanding cellular processes.
  • Existing single-molecule FISH (smFISH) methods are not established for whole-mount vertebrate organisms.

Purpose of the Study:

  • To establish and validate a novel smFISH method for whole-mount zebrafish embryos.
  • To enable quantitative analysis of gene expression noise in a vertebrate model organism.
  • To facilitate research on gene expression dynamics in development, physiology, and disease.

Main Methods:

  • Adaptation of smFISH for whole-mount zebrafish embryos.
  • Detection of single transcripts for cell-type specific and ubiquitous genes.

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Last Updated: Apr 17, 2026

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  • Demonstration of dual-gene detection in individual cells.

    • Main Results:

    • Successful implementation of smFISH in whole-mount zebrafish embryos.
    • Single-cell transcript detection achieved for various genes.
    • Simultaneous detection of two different genes demonstrated.

    Conclusions:

    • The developed whole-mount smFISH method is suitable for zebrafish embryos.
    • This technique provides a valuable tool for studying gene expression noise in a relevant vertebrate model.
    • Expected to advance research on gene expression, development, and disease in zebrafish.