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Updated: Apr 16, 2026

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
[Clusters of nonsolvent water in partially destroyed Saccharomyces cerevisiae yeast cells]
Abstract:
The state of water in partially destroyed dry yeast cells has been studied using low-temperature 1H NMR spectroscopy. It has been shown that the residual water is in the form of clusters of strongly and weakly associated water (SAW and WAW, respectively). Three or more types of SAW different in the chemical shift values have been found. It has been established that the interfacial water poorly dissolves hydrochloric and trifluoroacetic acids as well as DMSO and CD3CN. Hydrochloric acid on a surface of biomaterials can be separated into HCl and water. This process is stabilized by polar co-solvents (DMSO and CD3CN) added to the CDCl3 dispersion medium.
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