Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Epidemiology, clinical management, and outcomes in patients with eosinophilic granulomatosis with polyangiitis in England: A retrospective observational cohort study.

The journal of allergy and clinical immunology. Global·2026

Same author

Seven Years of Complaints from Patients in Plastic Surgery Hospitals About Autologous Fat Transfer Procedures.

Aesthetic plastic surgery·2026

Same author

Toward molecular and pathology-confirmed completeness in advanced ovarian cancer cytoreduction: Intraoperative molecular imaging and integrated theranostic strategies.

Molecular aspects of medicine·2026

Same author

Design, Synthesis, and Antiviral Evaluation of Novel 3,4-Dihydropyrimidin-2(1<i>H</i>)-one Derivatives.

Microorganisms·2026

Same author

Comprehensive 3D Analysis of Condyle After BSSRO in Surgery-First Approach Among Mandibular Prognathism Patients With and Without Asymmetry.

The Journal of craniofacial surgery·2026

Same author

Association between exposure to perfluoroalkyl ether sulfonate F-53B and cervical cancer risk and its mechanisms of cervical toxicity in Chinese women.

Ecotoxicology and environmental safety·2026

Search research articles

Related Experiment Video

Updated: Apr 16, 2026

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

Normalization and noise reduction for single cell RNA-seq experiments.

Bo Ding¹, Lina Zheng¹, Yun Zhu¹

¹Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, College of Computer Science and Technology, Jilin University, Changchun 130012, China and Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA.

Bioinformatics (Oxford, England)

|February 27, 2015

Summary

This summary is machine-generated.

This study introduces a novel method to reduce technical noise in single-cell RNA sequencing (scRNA-seq) data. The approach accurately estimates true gene expression levels, improving data interpretation.

More Related Videos

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

Related Experiment Videos

Last Updated: Apr 16, 2026

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Single-cell RNA sequencing (scRNA-seq) is crucial for understanding cellular heterogeneity.
Technical noise from limited input material hinders accurate scRNA-seq data interpretation.
Existing methods focus on differential gene expression, not direct noise reduction.

Purpose of the Study:

To develop a robust method for denoising scRNA-seq data.
To accurately compute true gene expression levels from noisy single-cell data.
To address the limitations of current scRNA-seq analysis techniques.

Main Methods:

A novel computational method was developed to remove technical noise.
The method utilizes External RNA Controls Consortium (ERCC) spike-in molecules.
The software is implemented in R and publicly available.

Main Results:

The proposed method effectively reduces technical noise in scRNA-seq data.
True gene expression levels are explicitly computed.
Improved accuracy in interpreting scRNA-seq data is achieved.

Conclusions:

The developed method offers a powerful and simple solution for scRNA-seq data denoising.
Accurate gene expression quantification is essential for reliable biological insights.
This approach enhances the utility of scRNA-seq for various biological applications.