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The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
A Shahrulazua1, Ar Samsudin2, Ma Iskandar3
1Department of Orthopaedics and Traumatology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Malaysian Orthopaedic Journal
|February 28, 2015
Summary
Sea cucumber extract (gamat) shows a concentration-dependent effect on human osteoblast cells. While high concentrations may inhibit viability, a specific low concentration (1mg/ml) significantly enhances it, suggesting potential therapeutic applications.
Area of Science:
- Biomedical Science
- Marine Biology
- Cell Biology
Background:
- Sea cucumbers, known as gamat in Malay, are utilized for their purported therapeutic benefits.
- The specific effects of gamat on human osteoblast cells remain largely uncharacterized.
- Osteoblasts are crucial for bone formation and health.
Purpose of the Study:
- To investigate the in vitro effects of Stichopus sp1 (gamat) extract on human osteoblast cell viability and function.
- To determine the dose-dependent relationship between gamat concentration and osteoblast response.
Main Methods:
- In vitro study utilizing human osteoblast cells.
- Treatment with varying concentrations of Stichopus sp1 extract.
- Assessment of cell viability using tetrazolium salts and evaluation of alkaline phosphatase activity.
Main Results:
- An inverse relationship was observed between gamat concentration and osteoblast cell viability (p<0.001).
- A concentration of 1mg/ml gamat significantly promoted cell viability at day 3.
- A trend towards increased osteoblast function was noted at 5mg/ml and 10mg/ml, but results were inconsistent across incubation periods.
Conclusions:
- Sea cucumber extract (gamat) exhibits a complex, concentration-dependent effect on human osteoblast cells.
- Low concentrations of gamat may enhance osteoblast viability, indicating potential for bone health applications.
- Further research is needed to clarify the inconsistent effects on osteoblast function at higher concentrations.
