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Published on: November 9, 2014
Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis
Meral Arslan Malkoç1, Necla Demİr1, Abdulkadir Şengün2
1Department of Prosthodontics, Faculty of Dentistry, İnönü University, Malatya, Turkey.
Purpose:
To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).
Materials And Methods:
Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 µL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments.
Results:
All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results.
Conclusion:
The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.
