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Updated: Apr 16, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
Published on: July 3, 2020
Short communication: Bovine-derived proteins activate STAT3 in human skeletal muscle in vitro
M K Caldow1, M R Digby2, D Cameron-Smith3
1Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne, 3125, Australia; Basic and Clinical Myology Laboratory, Department of Physiology, University of Melbourne, Melbourne, 3010, Australia.
Abstract:
Bovine milk contains biologically active peptides that may modulate growth and development within humans. In this study, targeted bovine-derived proteins were evaluated for their effects on signal transducer and activator of transcription-3 (STAT3) phosphorylation in human skeletal muscle cells. Following an acute exposure, bovine-derived acidic fibroblast growth factor-1 (FGF) and leukemia inhibitory factor (LIF) activated STAT3 in differentiating myotubes. Chronic exposure to FGF and LIF during the proliferative phase reduced myoblast proliferation and elevated MyoD and creatine kinase (CKM) mRNA expression without altering apoptotic genes. In mature myotubes, neither FGF nor LIF elicited any action. Together, these data indicate that a reduction in proliferation in the presence of bovine-derived FGF or LIF may stimulate early maturation of myoblasts.
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