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One-step surface modification of polyurethane using affinity binding peptides for enhanced fouling resistance
Yibing Wang1, Yong Yu, Liting Zhang
1a State Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center , East China University of Science and Technology , Shanghai 200237 , P.R. China.
Journal of Biomaterials Science. Polymer Edition
|March 4, 2015
Summary
Researchers discovered specific peptides that strongly bind to polyurethane (PU), enabling easy surface modification. These modified PU materials show reduced protein and cell adhesion, indicating potential for advanced biotechnological applications.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Peptide Engineering
Background:
- Synthetic polymers like polyurethane (PU) are widely used but often suffer from biofouling.
- Surface modification is crucial for enhancing the performance of polymeric materials in biomedical applications.
- Developing efficient methods for creating biocompatible and low-fouling surfaces is an ongoing challenge.
Purpose of the Study:
- To identify and characterize affinity binding peptides for polyurethane (PU).
- To explore the use of these peptides for facile surface modification of PU.
- To evaluate the impact of peptide functionalization on PU surface properties and biofouling resistance.
Main Methods:
- Biopanning of an M13 phage display peptide library against PU surfaces.
- Characterization of peptide binding affinity using apparent binding constant (K(app)) measurements.
- Analysis of peptide structure and binding mechanism, focusing on amino acid composition and hydrogen bonding.
- Assessment of modified PU surface properties, including wettability, protein adsorption, and cell adhesion.
Main Results:
- Discovery of peptides with high binding affinity to PU (K(app) up to 2.68 × 10(9) M(-1)).
- Achieved high surface peptide density exceeding 1.8 μg/cm(2).
- Identified peptides rich in H-donor amino acids, with hydrogen bonding as the primary binding driver.
- Demonstrated that peptide-treated PU exhibited increased wettability and significantly reduced protein adsorption and cell adhesion.
Conclusions:
- Affinity binding peptides can be effectively used for the surface functionalization of polyurethane.
- The identified peptides offer a simple and efficient one-step strategy for creating low-fouling polymeric surfaces.
- This approach holds promise for developing advanced biomaterials with enhanced biocompatibility for various biotechnological applications.
