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Updated: Apr 16, 2026
Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
Published on: August 25, 2017
[Expression of transient receptor potential canonical 1 in ozone-induced inflammatory lung tissues in mice]
Zhaodi Fu1, Lifen Zhou, Jianrong Huang
1Department of Physiology, Guangzhou Medical University, Guangzhou 510182, China.E-mail: fuzhaodi2013@tom.com.
Objective:
To detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of ozone-induced lung inflammation and explore its role in lung inflammation.
Methods:
In a mouse model of lung inflammation established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern blotting and immunohistochemistry.
Results:
Compared to the control mice, the mice exposed to ozone showed significantly increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed mice (P<0.01).
Conclusion:
TRPC1 may play a role in ozone-induced lung inflammation in mice.
