Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.8K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.8K
Histone Modification02:32

Histone Modification

17.8K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
17.8K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

9.1K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homocystinuria presenting with cerebral venous thrombosis: a case report highlighting progressive thrombosis.

Frontiers in cardiovascular medicine·2026
Same author

A Dual-Branch Deep Learning Framework with Explainability for Dental Caries Classification Using Intra-Oral Photographs and Radiographs.

Journal of imaging·2026
Same author

Effect of gelatin modification by sodium alginate and genipin cross-linking on gel properties and textural characterization.

Food chemistry·2026
Same author

Anti-Aging Protein Klotho Attenuates Schizophrenia-Associated Cognitive and Synaptic Dysfunctions by Regulating GluN2B-NMDARs in the Hippocampus.

Journal of neurochemistry·2026
Same author

Natural Products Inspired Scaffold Diversification Leads to Unnatural Molecular Warhead and Covalent Strategy to Modulating Protein Function through Electrophilic Bromine Transfer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

FXR and BET signaling orchestrate to protect β cells.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 16, 2026

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

11.4K

Methods to detect NF-κB acetylation and methylation.

JinJing Chen1, Lin-Feng Chen

  • 1Department of Biochemistry, College of Medicine, University of Illinois at Urbana-Champaign, COM 190 MSB, MC-714 506 S. Mathews Avenue, Urbana, IL, 61801, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2015
PubMed
Summary

Posttranslational modifications like acetylation and methylation regulate nuclear factor-kappa B (NF-κB) activity. New methods enable monitoring these NF-κB modifications in vitro and in vivo.

More Related Videos

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
07:13

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Published on: April 26, 2011

17.9K
A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

8.2K

Related Experiment Videos

Last Updated: Apr 16, 2026

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

11.4K
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
07:13

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Published on: April 26, 2011

17.9K
A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

8.2K

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Epigenetics

Background:

  • Nuclear factor-kappa B (NF-κB) is a key transcription factor regulating immune responses and cellular processes.
  • Posttranslational modifications, specifically lysine acetylation and methylation, critically control NF-κB's nuclear activity, duration, and transcriptional output.
  • The RelA subunit of NF-κB is extensively studied for its regulation by these modifications.

Purpose of the Study:

  • To describe experimental methods for monitoring NF-κB acetylation and methylation.
  • To provide techniques for analyzing these modifications both in vitro and in vivo.
  • To highlight the functional impact of specific lysine modifications on NF-κB.

Main Methods:

  • Radiolabeling of acetyl or methyl groups to validate modifications.
  • Immunoblotting using pan- or site-specific antibodies targeting acetylated or methylated lysine residues.
  • Application of these methods for in vitro and in vivo detection and functional analysis of NF-κB modifications.

Main Results:

  • Established methods for detecting and analyzing NF-κB acetylation and methylation.
  • Demonstrated the utility of radiolabeling for initial modification validation.
  • Showcased immunoblotting as a rapid and powerful tool for studying these modifications.

Conclusions:

  • Acetylation and methylation are crucial regulators of NF-κB function.
  • The described methods offer robust approaches to study NF-κB posttranslational modifications.
  • Understanding these modifications is vital for dissecting NF-κB-mediated cellular responses.