Related Experiment Video

Updated: Apr 16, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

15.1K

Sirt1 Regulates Microtubule Dynamics Through Negative Regulation of Plk1 in Mitosis

Jin-Ju Kim1, Na-Yeon Gil1, Xiang Hua Zhang2

  • 1College of Natural Sciences, Department of Life Sciences, Sogang University, Seoul, 121-742, Republic of Korea.

Journal of Cellular Biochemistry
|March 5, 2015
PubMed

Insights

Loss of Sirt1 impairs mitotic spindle formation and chromosome segregation by affecting Plk1 activity. This study reveals Sirt1

Area of Science:

  • Molecular biology
  • Cell biology
  • Genetics

Background:

  • Sirtuin 1 (Sirt1) loss is linked to chromosome aneuploidy and increased tumor susceptibility.
  • The precise molecular mechanisms underlying this link are not fully understood.

Purpose of the Study:

  • To investigate the role of Sirt1 in regulating mitotic progression and chromosome segregation.
  • To elucidate the molecular mechanisms by which Sirt1 influences spindle dynamics.

Main Methods:

  • Investigated the direct regulation of Polo-like kinase 1 (Plk1) by Sirt1.
  • Examined the effects of Sirt1 depletion or inhibition on mitotic spindle formation and chromosome segregation.
  • Assessed Plk1 activity and microtubule dynamics in Sirt1-deficient cells.

Main Results:

  • Sirt1 directly regulates the activity of Plk1, a key regulator of mitosis.
  • Loss of Sirt1 function leads to significant defects in mitotic spindle assembly and chromosome segregation.
  • Elevated depolymerization of the mitotic spindle in Sirt1-deficient cells correlates with Plk1 deregulation.

Conclusions:

  • Sirt1 acts as a crucial mitotic regulator, controlling spindle dynamics via Plk1 activity.
  • Sirt1 fine-tunes Plk1-dependent microtubule dynamics, thereby ensuring accurate chromosome segregation.
  • Understanding this Sirt1-Plk1 axis may offer new insights into cancer development and treatment.
Keywords:
MICROTUBULE DYNAMICSMITOTIC ARRESTNOCODAZOLEPlk1Sirt1

More Related Videos

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

3.2K
Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

6.7K

Related Concept Videos

Destabilization of Microtubules01:45

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.9K
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
6.5K
Microtubule Instability02:17

Microtubule Instability

6.3K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.3K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.8K