Hypoxia/hypotension evoked release of glutamate and aspartate from the rat cerebral cortex

Glutamate and aspartate levels in cerebral cortical superfusates rose rapidly after introduction of artificial cerebrospinal fluid into cortical cups to reach stable levels within 5-10 min. These equilibrated levels (glutamate 1.33 +/- 0.1 microM; aspartate 0.29 +/- 0.04 microM), which are very similar to those in fourth ventricular CSF (glutamate, 1.5 +/- 0.2 microM; aspartate, 0.27 +/- 0.03 microM), are likely to reflect basal interstitial amino acid levels in the superficial layers of the cortex. During and following brief (5 min) hypoxemic/hypotensive episodes, release of both excitatory amino acids into the cortical superfusates was enhanced. The finding of an hypoxia/hypotension-evoked release of excitatory amino acids is consistent with recent speculation that these agents may play an important role in the destruction of neurons in ischemic regions of the brain.