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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

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Innovations in air sampling to detect plant pathogens.

Js West¹, Rbe Kimber²

¹Department of Plant Biology and Crop Science, Rothamsted Research St Albans, UK.

The Annals of Applied Biology

|March 7, 2015

Summary

Innovations in air sampling technology enhance plant disease management and detection. Advanced devices improve pathogen identification, dispersal prediction, and precision agriculture for better crop protection.

Keywords:

Aerobiology GIS IDM IPM UAV cyclone diagnostics virtual impactor

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Area of Science:

Plant Pathology
Aerobiology
Agricultural Technology

Background:

The Hirst spore trap marked an early milestone in air sampling for plant pathology.
Significant advancements have since been made in device efficiency, sample processing, and automation.

Purpose of the Study:

To review innovations in air sampling devices for plant pathology.
To highlight advancements in automation, mobile platforms, and data integration for disease management.

Main Methods:

Review of technological developments in air sampling devices.
Integration of Geographical Information Systems (GIS) with sampler networks.
Application of novel diagnostic methods for rapid quantification of airborne species.

Main Results:

Improved capture efficiency and automated sample processing.
Deployment of air samplers on mobile platforms (UAVs, ground vehicles) for dynamic sampling.
Enhanced prediction of airborne inoculum and dispersal dynamics using GIS networks.

Conclusions:

Air sampling technology is rapidly evolving with novel diagnostics for accurate quantification.
Understanding dispersal processes improves precision in crop protection and integrated pest management.
Increased adoption by growers and industry is expected, with applications extending to allergen detection and regional disease dynamics.