Estimating effective population size from temporally spaced samples with a novel, efficient maximum-likelihood...

The effective population size [Formula: see text] is a key parameter in population genetics and evolutionary biology, as it quantifies the expected distribution of changes in allele frequency due to genetic drift. Several methods of estimating [Formula: see text] have been described, the most direct of which uses allele frequencies measured at two or more time points. A new likelihood-based estimator [Formula: see text] for contemporary effective population size using temporal data is developed in this article. The existing likelihood methods are computationally intensive and unable to handle the case when the underlying [Formula: see text] is large. This article tries to work around this problem by using a hidden Markov algorithm and applying continuous approximations to allele frequencies and transition probabilities. Extensive simulations are run to evaluate the performance of the proposed estimator [Formula: see text], and the results show that it is more accurate and has lower variance than previous methods. The new estimator also reduces the computational time by at least 1000-fold and relaxes the upper bound of [Formula: see text] to several million, hence allowing the estimation of larger [Formula: see text]. Finally, we demonstrate how this algorithm can cope with nonconstant [Formula: see text] scenarios and be used as a likelihood-ratio test to test for the equality of [Formula: see text] throughout the sampling horizon. An R package "NB" is now available for download to implement the method described in this article.