Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Exon Recombination02:32

Exon Recombination

4.3K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.3K
Epistasis Analysis01:09

Epistasis Analysis

6.3K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
6.3K
Epistasis01:39

Epistasis

51.6K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
51.6K
Ribosome Profiling02:24

Ribosome Profiling

4.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.4K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

17.4K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
17.4K
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

72
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
72

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A NECAP1 Nonsense Variant is Associated With Leukoencephalomyelopathy With Oligodendroglial Dysplasia in a Belgian Malinois With Spinocerebellar Ataxia.

Animal genetics·2026
Same author

Impact of cardiometabolic comorbidities on clinical characteristics, prescription patterns and retention rate of first b/tsDMARD treatment in 5299 European real-world patients with psoriatic arthritis.

RMD open·2026
Same author

National Surveillance of Candidemia in the Czech Republic: Preliminary Results from the 2023 Multicentre Study.

Mycopathologia·2026
Same author

Comparative evaluation of Anyplex™ MTB/NTM, Xpert MTB/RIF ultra, culture, and microscopy for the diagnosis of pediatric tuberculosis using gastric aspirates.

Annals of clinical microbiology and antimicrobials·2026
Same author

Tuberculosis transmission and resistance among Ukrainian migrants and its impact on multidrug-resistant tuberculosis dynamics in high-influx host countries.

BMC microbiology·2026
Same author

Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria.

Nucleic acids research·2025

Related Experiment Video

Updated: Apr 16, 2026

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

2.8K

Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression.

Kateřina Svobodová1, Pavel Horák, Antonín Stratil

  • 1Department of Morphology, Physiology and Animal Genetics, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic.

Molecular Biology Reports
|March 10, 2015
PubMed
Summary

This study compares porcine eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) and EEF1A2 genes, detailing their genomic sequences, organization, expression patterns, and chromosomal locations. Novel polymorphisms were identified in both genes.

More Related Videos

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

4.5K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.7K

Related Experiment Videos

Last Updated: Apr 16, 2026

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

2.8K
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

4.5K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.7K

Area of Science:

  • Genomics
  • Molecular Biology
  • Animal Genetics

Background:

  • Eukaryotic translation elongation factor 1 alpha (EEF1A) is crucial for protein synthesis.
  • Higher vertebrates possess two EEF1A isoforms: EEF1A1 and EEF1A2, encoded by separate genes.

Purpose of the Study:

  • To compare porcine EEF1A1 and EEF1A2 genes regarding genomic sequence, organization, and mRNA expression.
  • To identify polymorphisms and determine chromosomal assignments for these porcine genes.

Main Methods:

  • Standard DNA and mRNA analysis techniques were employed.
  • Genomic sequencing, gene organization analysis, and mRNA expression profiling across various tissues were performed.
  • Chromosomal mapping was conducted using established methods.

Main Results:

  • Complete genomic sequences for porcine EEF1A1 and EEF1A2 were determined, revealing differences in transcription unit lengths but similar genomic organization.
  • Coding sequences showed 78% identity, and amino acid sequences exhibited 92.4% identity.
  • Novel polymorphisms were detected in both genes, and EEF1A1 and EEF1A2 were mapped to SSC1p11.1 and SSC17q23.3, respectively.
  • EEF1A1 mRNA was ubiquitously expressed, with highest levels in fetal muscle, while EEF1A2 showed restricted expression in adult skeletal muscle, tongue, heart, diaphragm, and brain, notably absent in 44-day fetal muscle.

Conclusions:

  • This study provides comprehensive data on porcine EEF1A1 and EEF1A2, including their genomic characteristics, expression profiles, and chromosomal locations.
  • The identification of novel polymorphisms offers valuable genetic markers.
  • This research marks the first detailed study of porcine EEF1A2, contributing significantly to the understanding of translation elongation factors in pigs.